Applications Of Lc Ms In Toxicology

Autor: Aldo Polettini
Publisher: Pharmaceutical Press
ISBN: 9780853696292
File Size: 65,48 MB
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Univ. of Pavia, Italy. Covers all essential theoretical aspects of LC-MS, including technical details of the instrumentation and different operating modes, method development, optimisation and validation, quantification, and criteria for identification and confirmation. For forensic and clinical toxicologists.

Lc Ms In Drug Analysis

Autor: Loralie J. Langman
Publisher: Humana Press
ISBN: 9781493961993
File Size: 45,12 MB
Format: PDF
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Liquid-Chromatography-Mass-Spectrometry procedures have been shown to be successful when applied to drug development and analysis. LC-MS in Drug Analysis: Methods and Protocols provides detailed LC-MS/MS procedures for the analysis of several compounds of clinical significance. The first chapters provide the reader with an overview of mass spectroscopy, its place in clinical practice, its application of MS to TDM and toxicology, and the merits of LC-MS(/MS) and new sample preparation techniques. The following chapters discuss different approaches to screening for drugs of abuse and for general unknowns, as well as targeted measurement of specific analytes or classes of analytes including abused drugs, toxic compounds, and therapeutic agents. Written in the successful Methods in Molecular BiologyTM series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and easily accessible, LC-MS in Drug Analysis: Methods and Protocols seeks to serve both professionals and novices with its well-honed methodologies.

Lc Ms

Autor: Marvin C. McMaster
Publisher: John Wiley & Sons
ISBN: 0471736570
File Size: 14,98 MB
Format: PDF, Kindle
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A practical guide to using and maintaining an LC/MS system The combination of liquid chromatography (LC) and mass spectrometry (MS) has become the laboratory tool of choice for a broad range of industries that require the separation, analysis, and purification of mixtures of organic compounds. LC/MS: A Practical User's Guide provides LC/MS users with an easy-to-use, hands-on reference that focuses on the practical applications of LC/MS and introduces the equipment and techniques needed to use LC/MS successfully. Following a thorough explanation of the basic components and operation of the LC/MS system, the author presents empirical methods for optimizing the techniques, maintaining the instrumentation, and choosing the appropriate MS or LC/MS analyzer for any given problem. LC/MS covers everything users need to know about: The latest equipment, including quadrupole, time-of-flight, and ion trap analyzers Cutting-edge processes, such as preparing HPLC mobile phases and samples; handling and maintaining a wide variety of silica, zirconium, and polymeric separation columns; interpreting and quantifying mass spectral data; and using MS interfaces Current and future applications in the pharmaceutical and agrochemical industries, biotechnology, clinical research, environmental studies, and forensics An accompanying PowerPoint® slide-set on CD-ROM provides vital teaching tools for instructors and new equipment operators. Abundantly illustrated and easily accessible, the text is designed to help students and practitioners acquire optimum proficiency in this powerful and rapidly advancing analytical application.

Mass Spectrometry For The Clinical Laboratory

Autor: Hari Nair
Publisher: Academic Press
ISBN: 0128009926
File Size: 30,46 MB
Format: PDF
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Mass Spectrometry for the Clinical Laboratory is an accessible guide to mass spectrometry and the development, validation, and implementation of the most common assays seen in clinical labs. It provides readers with practical examples for assay development, and experimental design for validation to meet CLIA requirements, appropriate interference testing, measuring, validation of ion suppression/matrix effects, and quality control. These tools offer guidance on what type of instrumentation is optimal for each assay, what options are available, and the pros and cons of each. Readers will find a full set of tools that are either directly related to the assay they want to adopt or for an analogous assay they could use as an example. Written by expert users of the most common assays found in a clinical laboratory (clinical chemists, toxicologists, and clinical pathologists practicing mass spectrometry), the book lays out how experts in the field have chosen their mass spectrometers, purchased, installed, validated, and brought them on line for routine testing. The early chapters of the book covers what the practitioners have learned from years of experience, the challenges they have faced, and their recommendations on how to build and validate assays to avoid problems. These chapters also include recommendations for maintaining continuity of quality in testing. The later parts of the book focuses on specific types of assays (therapeutic drugs, Vitamin D, hormones, etc.). Each chapter in this section has been written by an expert practitioner of an assay that is currently running in his or her clinical lab. Provides readers with the keys to choosing, installing, and validating a mass spectrometry platform Offers tools to evaluate, validate, and troubleshoot the most common assays seen in clinical pathology labs Explains validation, ion suppression, interference testing, and quality control design to the detail that is required for implementation in the lab

Fundamentals Of Analytical Toxicology

Autor: Robert J. Flanagan
Publisher: John Wiley & Sons
ISBN: 0470516283
File Size: 53,41 MB
Format: PDF, ePub
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The analytical toxicologist may be required to detect, identify, and in many cases measure a wide variety of compounds in samples from almost any part of the body or in related materials such as residues in syringes or in soil. This book gives principles and practical information on the analysis of drugs and poisons in biological specimens, particularly clinical and forensic specimens. After providing some background information the book covers aspects of sample collection, transport, storage and disposal, and sample preparation. Analytical techniques - colour tests and spectrophotometry, chromatography and electrophoresis, mass spectrometry, and immunoassay – are covered in depth, and a chapter is devoted to the analysis of trace elements and toxic metals. General aspects of method implementation/validation and laboratory operation are detailed, as is the role of the toxicology laboratory in validating and monitoring the performance of point of care testing (POCT) devices. The book concludes with reviews of xenobiotic absorption, distribution and metabolism, pharmacokinetics, and general aspects of the interpretation of analytical toxicology results. A clearly written, practical, integrated approach to the basics of analytical toxicology. Focuses on analytical, statistical and pharmacokinetic principles rather than detailed applications. Assumes only a basic knowledge of analytical chemistry. An accompanying website provides additional material and links to related sites. Written by an experienced team of authors,Fundamentals of Analytical Toxicology is an invaluable resource for those starting out in a career in analytical toxicology across a wide range of disciplines including clinical and forensic science, food safety, and pharmaceutical development. Praise from the reviews: “This is an ambitious effort to describe in detail the many and varied aspects of the science of toxicological analysis. The 17 chapters cover every foreseeable aspect, from specimen collection through analytical techniques and quality control to pharmacological principles and interpretation of results. The authors bring together a great deal of experience in the field and have succeeded admirably in achieving their goal: "to give principles and practical information on the analysis of drugs, poisons and other relevant analytes in biological specimens...". The book is very readable and quite up-to-date, and contains many illustrative figures, charts and tables. Both the student and the practicing professional would do well to study this material carefully, as there is something here for every conceivable level of interest.” Review from Randall Baselt "This text comes highly recommended for any analytical toxicology trainee." The Bulletin of the Royal College of Pathologists “Overall, this book provides a comprehensive, thorough, clear, up to date and practical treatment of analytical toxicology at a high standard. Understanding of the text is enhanced by the use of many illustrations. Specifications, guidelines, and methods are highlighted in grey background “Boxes”. The many and up to date literature references in each chapter demonstrate the authors’ thorough work and permit easy access to deeper information. Therefore this book can be highly recommended as a valuable source of knowledge in analytical toxicology both as an introduction and for the advanced reader.” GTFCh Bulletin “Toxichem + Krimtech”, May 2008 (translated, original review in German) “Many toxicologists will add this important reference to their libraries because it competently fills a need ...” International Journal of Toxicology

Mass Spectrometry In Drug Discovery

Autor: David T. Rossi
Publisher: CRC Press
ISBN: 9781420002478
File Size: 54,61 MB
Format: PDF, ePub
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Mass Spectrometry in Drug Discovery summarizes the theory, instrumentation, techniques, and application of mass spectrometry and atmospheric pressure ionization to screening, evaluating, and improving the performance and quality of drug candidates. It provides time- and cost-efficient approaches for the generation and analysis of effective pharmaceuticals, covers advances in combinatorial chemistry, molecular biology, bioanalysis automation, and computing, and demonstrates the use of mass spectrometry in the assessment of disease states, drug targets, and potential drug agents.

Chromatographic Methods In Clinical Chemistry And Toxicology

Autor: Roger Bertholf
Publisher: John Wiley & Sons
ISBN: 0470023104
File Size: 76,83 MB
Format: PDF, Docs
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This book aims to fill the gap that exists between theoretical treatments of chromatography, and clinical chemistry and toxicology texts, which focus almost exclusively on clinical relevance and applications. Chromatography has a vast array of clinical applications, and though the chromatographic methods were first introduced decades ago, new applications of this technology are being used to explore previously inaccessible frontiers in clinical diagnostics and toxicological testing. An up-to-date book devoted to clinical and toxicological applications of chromatographic methods will serve as an instructional and reference text, useful to students, laboratory technicians, and researchers.

Clarke S Analytical Forensic Toxicology

Autor: Gail Cooper
Publisher: Pharmaceutical Press
ISBN: 0857110543
File Size: 56,57 MB
Format: PDF, Docs
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Clarke's Analytical Forensic Toxicology offers a fresh perspective on the drugs and poisons that are most likely to be encountered in forensic toxicology. It focuses on collection, extraction and analysis. This second edition is fully updated to reflect advances in analytical and forensic toxicology. New and extended chapters include: sampling, storage and stability; in-utero exposure to drugs of abuse; drug-facilitated sexual assault; extraction. Additional features are included from the fourth edition of Clarke's Analysis of Drugs and Poisons.

Advances In Forensic Applications Of Mass Spectrometry

Autor: Jehuda Yinon
Publisher: CRC Press
ISBN: 9780203998281
File Size: 20,39 MB
Format: PDF
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Recent developments in analytical instrumentation have had an enormous influence on forensic analysis. The mass spectrometer is now an integral part of every forensic laboratory, resulting in greater analytical accuracy, more reliable identification, and lower detection limits. As the instrumental method of choice among forensic analysts, the mass spectrometer continues to pave the way for innovative applications in forensic analysis. Exploring the latest developments in the field, Advances in Forensic Applications of Mass Spectrometry provides a comprehensive reference guide to forensic applications using modern mass spectrometry techniques. The book features methods developed from advanced instrumentation, drug detection and toxicological analysis, and methodology of applications of stable isotope ratios. The final chapters present approaches for identifying accelerants in fire debris and procedures for analyzing explosives in post-blast residues. Jehuda Yinon, Ph.D., is one of the top forensic chemists in the world. As the editor of this authoritative volume, he compiles the knowledge of leading experts to demonstrate the use of novel mass spectrometry techniques in a vast range of forensic applications.